Neutralization and Decontamination Station in Stráž pod Ralskem and Sludge Storage

Tato bakalářská práce se zabývá popisem technologií neutralizačně dekontaminační stanice na zpracování matečných louhů ve městě Stráž pod Ralskem ve státním podniku Diamo odštěpný závod Těžba a úprava uranu. Technologie spočívá v čerpání zbytkových technologických roztoků na stanici, kde probíhá jejich neutralizace pomocí vápenného mléka. Technologie se skládá z několika provozních souborů jako jsou neutralizace, filtrace, vápenné hospodářství, stripování, absorpce amoniaku, čpavkové hospodářství atd. Dále se tato práce zabývá odkalištěm, které se nachází ve Stráži pod Ralskem a které je přímo situované u neutralizačně dekontaminační stanice na zpracování matečných louhů. V rámci odkaliště je zde popsána jeho základní popis, poloha, rozdělení na etapy, rozepsány hydrogeologické poměry, systém hrází, čerpací stanice.This bachelor's thesis deals with the description of the technologies of the neutralization decontamination station for the processing of mother liquors in the town of Stráž pod Ralskem in the state enterprise Diamo branch plant Těžba a úprava uranu. The technology consists in pumping residual technological solutions at the station, where they are neutralized with whitening. The technology consists of several operational files such as neutralization, filtration, lime management, stripping, ammonia absorption, ammonia management, etc. Furthermore, this work deals with the sludge pond, which is located in Stráž pod Ralskem and which is directly located at the neutralization decontamination station for the processing of mother liquors. Within the sludge pond, its basic description, location, division into stages, hydrogeological conditions, dam system, pumping station are described.542 - Katedra hornického inženýrství a bezpečnostidobř

Coexistence of underground and chemical exploitation at Stráž pod Ralskem

Předložená diplomová práce podrobně hodnotí přístupy dvou odlišných dobývacích metod, které koexistovaly na velmi malé ploše, na lokalitě Stráž pod Ralskem, kde se jimi dobýval uran. Jednalo se o klasickou hlubinnou metodu a metodu podzemního vyluhování. Diplomová práce spočívá v hodnocení dobývacích metod, jejich vývoji, problematikou, kterou tyto metody v koexistování měly, a hlavně hodnocení jejich vlivů na životní prostředí v blízkém okolí těžby. Dále se zde seznámíme s geolokalitou zájmové oblasti, ekonomickými náročnostmi a politickými rozhodnutími, které zde vedly k rozvoji dobývání uranu. Na závěr předložené diplomové práce se seznámíme se sanačními technologiemi a podíváme se na sanaci horninového prostředí, jež je nutná k zahlazení následků hornické činnosti a zhodnotíme, zda by k rozsahu sanačních prací došlo, pokud by jednotlivé metody nekoexistovaly.The presented diploma thesis evaluates in detail the approaches of two different mining methods that coexisted on a very small area, at the locality Stráž pod Ralskem, where uranium was mined by these two different methods. These were the classic deep mining method and the underground leaching method. The diploma thesis consists in the evaluation of mining methods, their development, the problems that these methods had in coexistence, and mainly the evaluation of their effects on the environment in the immediate vicinity of mining. Furthermore, we will get to know the geographical location of the area of interest, the economic challenges and political decisions that led to the development of uranium mining here. At the end of the presented diploma thesis, we will familiarize ourselves with remediation technologies and look at the remediation of the rock environment, which is necessary to smooth out the consequences of mining activity, and we will evaluate whether the extent of remediation work would occur if the individual methods did not coexist.542 - Katedra hornického inženýrství a bezpečnostivýborn

IRESite: the database of experimentally verified IRES structures ()

IRESite is an exhaustive, manually annotated non-redundant relational database focused on the IRES elements (Internal Ribosome Entry Site) and containing information not available in the primary public databases. IRES elements were originally found in eukaryotic viruses hijacking initiation of translation of their host. Later on, they were also discovered in 5′-untranslated regions of some eukaryotic mRNA molecules. Currently, IRESite presents up to 92 biologically relevant aspects of every experiment, e.g. the nature of an IRES element, its functionality/defectivity, origin, size, sequence, structure, its relative position with respect to surrounding protein coding regions, positive/negative controls used in the experiment, the reporter genes used to monitor IRES activity, the measured reporter protein yields/activities, and references to original publications as well as cross-references to other databases, and also comments from submitters and our curators. Furthermore, the site presents the known similarities to rRNA sequences as well as RNA–protein interactions. Special care is given to the annotation of promoter-like regions. The annotated data in IRESite are bound to mostly complete, full-length mRNA, and whenever possible, accompanied by original plasmid vector sequences. New data can be submitted through the publicly available web-based interface at and are curated by a team of lab-experienced biologists

IRESite—a tool for the examination of viral and cellular internal ribosome entry sites

The IRESite (http://www.iresite.org) presents carefully curated experimental evidence of many eukaryotic viral and cellular internal ribosome entry site (IRES) regions. At the time of submission, IRESite stored >600 records. The IRESite gradually evolved into a robust tool providing (i) biologically meaningful information regarding the IRESs and their experimental background (including annotation of IRES secondary structures and IRES trans-acting factors) as well as (ii) thorough concluding remarks to stored database entries and regularly updated evaluation of the reported IRES function. A substantial portion of the IRESite data results purely from in-house bioinformatic analyses of currently available sequences, in silico attempts to repeat published cloning experiments, DNA sequencing and restriction endonuclease verification of received plasmid DNA. We also present a newly implemented tool for displaying RNA secondary structures and for searching through the structures currently stored in the database. The supplementary material contains an updated list of reported IRESs

Genome fractionation and loss of heterozygosity in hybrids and polyploids: Mechanisms, consequences for selection, and link to gene function

Hybridization and genome duplication have played crucial roles in the evolution of many animal and plant taxa. The subgenomes of parental species undergo considerable changes in hybrids and polyploids, which often selectively eliminate segments of one subgenome. However, the mechanisms underlying these changes are not well understood, particularly when the hybridization is linked with asexual reproduction that opens up unexpected evolutionary pathways. To elucidate this problem, we compared published cytogenetic and RNAseq data with exome sequences of asexual diploid and polyploid hybrids between three fish species; Cobitis elongatoides, C taenia, and C tanaitica. Clonal genomes remained generally static at chromosome-scale levels but their heterozygosity gradually deteriorated at the level of individual genes owing to allelic deletions and conversions. Interestingly, the impact of both processes varies among animals and genomic regions depending on ploidy level and the properties of affected genes. Namely, polyploids were more tolerant to deletions than diploid asexuals where conversions prevailed, and genomic restructuring events accumulated preferentially in genes characterized by high transcription levels and GC-content, strong purifying selection and specific functions like interacting with intracellular membranes. Although hybrids were phenotypically more similar to C taenia, we found that they preferentially retained C elongatoides alleles. This demonstrates that favored subgenome is not necessarily the transcriptionally dominant one. This study demonstrated that subgenomes in asexual hybrids and polyploids evolve under a complex interplay of selection and several molecular mechanisms whose efficiency depends on the organism's ploidy level, as well as functional properties and parental ancestry of the genomic region.Web of Science38125274525

EuReCa ONE—27 Nations, ONE Europe, ONE Registry A prospective one month analysis of out-of-hospital cardiac arrest outcomes in 27 countries in Europe

AbstractIntroductionThe aim of the EuReCa ONE study was to determine the incidence, process, and outcome for out of hospital cardiac arrest (OHCA) throughout Europe.MethodsThis was an international, prospective, multi-centre one-month study. Patients who suffered an OHCA during October 2014 who were attended and/or treated by an Emergency Medical Service (EMS) were eligible for inclusion in the study. Data were extracted from national, regional or local registries.ResultsData on 10,682 confirmed OHCAs from 248 regions in 27 countries, covering an estimated population of 174 million. In 7146 (66%) cases, CPR was started by a bystander or by the EMS. The incidence of CPR attempts ranged from 19.0 to 104.0 per 100,000 population per year. 1735 had ROSC on arrival at hospital (25.2%), Overall, 662/6414 (10.3%) in all cases with CPR attempted survived for at least 30 days or to hospital discharge.ConclusionThe results of EuReCa ONE highlight that OHCA is still a major public health problem accounting for a substantial number of deaths in Europe.EuReCa ONE very clearly demonstrates marked differences in the processes for data collection and reported outcomes following OHCA all over Europe. Using these data and analyses, different countries, regions, systems, and concepts can benchmark themselves and may learn from each other to further improve survival following one of our major health care events

Use of clay minerals to produce biodegradable mixtures based on polyvinyl alcohol and keratin hydrolyzate

In the research, composite PVA films to contain selected types of clay minerals and keratin hydrolyzate were designed. The addition of clay Fillem can also improve the process and user properties of the resulting composite. In the research the Cloisite® Na+, Cloisite® 20A, Cloisite® 30B, Kaolin Sedlec 1A, waste Kaolin Strelec and zeolites prepared by synthesis of the waste materials. The blended films were subsequently subjected to biodegradation experiments. The test results indicate the effect of the mineral clay type on the resulting film decomposition

Firefly luciferase gene contains a cryptic promoter

A firefly luciferase (FLuc) counts among the most popular reporters of present-day molecular and cellular biology. In this study, we report a cryptic promoter activity in the luc+ gene, which is the most frequently used version of the firefly luciferase. The FLuc coding region displays cryptic promoter activity both in mammalian and yeast cells. In human CCL13 and Huh7 cells, cryptic transcription from the luc+ gene is 10–16 times weaker in comparison to the strong immediate-early cytomegalovirus promoter. Additionally, we discuss a possible impact of the FLuc gene cryptic promoter on experimental results especially in some fields of the RNA-oriented research, for example, in analysis of translation initiation or analysis of miRNA/siRNA function. Specifically, we propose how this newly described cryptic promoter activity within the FLuc gene might contribute to the previous determination of the strength of the cryptic promoter found in the cDNA corresponding to the hepatitis C virus internal ribosome entry site. Our findings should appeal to the researchers to be more careful when designing firefly luciferase-based assays as well as open the possibility of performing some experiments with the hepatitis C virus internal ribosome entry site, which could not be considered until now

transXpress: a Snakemake pipeline for streamlined de novo transcriptome assembly and annotation

Abstract Background RNA-seq followed by de novo transcriptome assembly has been a transformative technique in biological research of non-model organisms, but the computational processing of RNA-seq data entails many different software tools. The complexity of these de novo transcriptomics workflows therefore presents a major barrier for researchers to adopt best-practice methods and up-to-date versions of software. Results Here we present a streamlined and universal de novo transcriptome assembly and annotation pipeline, transXpress, implemented in Snakemake. transXpress supports two popular assembly programs, Trinity and rnaSPAdes, and allows parallel execution on heterogeneous cluster computing hardware. Conclusions transXpress simplifies the use of best-practice methods and up-to-date software for de novo transcriptome assembly, and produces standardized output files that can be mined using SequenceServer to facilitate rapid discovery of new genes and proteins in non-model organisms

Verification of diffusion mathematical model for long-term materials drying

The paper deals with a design of a diffusion mathematical model describing the drying processes for longlasting desiccation of dried substances prototypically shaped as plane, cylinder, and sphere. A decrease in the substance humidity shrinkage is derived from the result in deterministic model of desiccation. The long-term desiccation of the dried substances has been simulated on the basis of COMSOL MULTIPHYSICS programme and MAPLE, where initial and marginal conditions were defined. The functional program application in MATLAB has been created in order to draw a contrast among particular results obtained from the COMSOL programme.The application enables delineating the moisture extent in substances, 3D graph of moisture extent, and a decrease in the substance humidity degree. By means of the mentioned application, it is achievable to evaluate the results even in terms of changes in some of the set parameters. The shrinkage in substance moisture can be predicted in relation to suggested mathematical models. This fact has been confirmed bythe results of long-lasting desiccation of green coffee-beans in the warehouse